Simplified Homogeneous Fluid Medium for Tubercle Bacilli

Pyruvic acid egg medium for tubercle bacilli.

Sputum culture for tubercle bacilli on a solid egg medium containing glycerol was compared with culture on a similar medium containing pyruvic acid and no glycerol. Tubercle bacilli from the sputum of five out of 99 patients grew on pyruvic acid medium but not on glycerol medium. The addition of pyruvic acid is therefore essential when an egg medium is used for the cultural diagnosis of tubercu...

Simplified Fluorescence Microscopy of Tubercle Bacilli

The fluorescence of tubercle bacilli stained with auramine and rhodamine does not require ultra-violet light. It can be caused by blue light up to 406mp. A’copper sdphate ammonia liquid filter, suitably diluted, will transmit this waveband. A gelatin screen filter, absorbing all light below 510mp. is used in the eyepiece. Normal, high intensity projection Alament lamps, combined with lamp conde...

Cultivation of Tubercle Bacilli from Pleural Fluid

The diand tri-peptidase activity in white blood cells showed little variation from day to day (Table 11). This variation bore no relation to the changes in the number of white blood cells per c.mm. observed in cases of leukaemia. However, the activity of the enzymes differed in the diverse types ofwhite blood cells examined. White blood cells from cases of acute and chronic myeloid leukaemias g...

A Simple Medium for Growth of Tubercle Bacilli *

Blood as a medium for the cultivation of tubercle bacilli was first employed successfully by Koch.' Pryce2 in 1941 reported the use of sputum film cultures suspended in lysed blood, and suggested this technique for pus and caseous material. The medium used in the present study consists of citrated human blood lysed with equal parts of a one per cent solution of saponin in water, to which glycer...

Stomach-Wash Examinations for Tubercle Bacilli